primary human bronchial epithelial cells hbepc  (PromoCell)

Journal: Neoplasia (New York, N.Y.)

Article Title: Multi-omics profiling reveals microenvironmental remodeling as a key driver of house dust mite-induced lung cancer progression

doi: 10.1016/j.neo.2026.101275

Figure Lengend Snippet: HDM d oes not have mutagenic effects in human and mouse lung epithelial cell lines i n v itro . A) Dose-response curves of HDM treatment in BEAS-2B and LLC cells. Cell viability is shown relative to vehicle control (PBS; % of control). Calculated IC 50 values are indicated. B) Experimental setup for HDM exposure and duplex sequencing. BEAS-2B and LLC cells were treated with either HDM (200 µg/mL for BEAS-2B; 400 µg/mL for LLC) or vehicle (VEH; PBS), expanded across multiple passages, and subsequently subjected to genomic sequencing. C) Comparison of mutational burden between samples treated with either HDM (purple) or VEH (blue), shown as somatic mutations per megabase (Mb) of coding DNA. D) Mutational spectra of BEAS-2B and LLC cells treated with HDM compared to VEH controls. Mutations are classified into the six base substitution categories and displayed in SBS96 format. The experiments were performed in triplicate (A) or singlicate (B-D).

Article Snippet: Human bronchial epithelial cells (BEAS-2B) were purchased in 2018 from ATCC (Cat. No CRL-9609).

Techniques: Control, Sequencing, Genomic Sequencing, Comparison

Journal: Materials Today Bio

Article Title: Polystyrene nanoplastics-induced lung epithelial cells ferroptosis promotes pulmonary fibrosis via YY1/FTL axis

doi: 10.1016/j.mtbio.2025.102738

Figure Lengend Snippet: PS-NPs-Induced Ferroptosis in BEAS-2B Cells and Paracrine Fibroblast Activation: (A–B) Western blot of GPX4 and FTL in BEAS-2B cells after 24 h exposure to PS-NPs at 0, 50, 100, and 200 μg/ml (C–D) Time-course of GPX4 and FTL expression in cells treated with 200 μg/ml PS-NPs for 0, 3, 6, 12, and 24 h. (E) Transmission electron microscopy images of control and PS-NPs treated BEAS-2B cells showing characteristic mitochondrial shrinkage. (F–G) Restoration of GPX4 following co-treatment with Fer-1. (H) C11-BODIPY581/591 fluorescence showing Fer-1–mediated attenuation of lipid peroxidation. (I) FerroOrange staining demonstrating reduction of labile Fe 2+ upon Fer-1 co-incubation. (J–O) COL1 and α-SMA mRNA (qPCR) and protein (western blot) levels in MRC-5 fibroblasts after 24 h treatment with conditioned medium from BEAS-2B cells exposed to PS-NPs ± Fer-1. Statistical significance is denoted as *p < 0.05 and **p < 0.01.

Article Snippet: The human bronchial epithelial cell (BEAS-2B) and human embryonic lung fibroblast (MRC-5) were commercially purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Activation Assay, Western Blot, Expressing, Transmission Assay, Electron Microscopy, Control, Fluorescence, Staining, Incubation

Journal: Materials Today Bio

Article Title: Polystyrene nanoplastics-induced lung epithelial cells ferroptosis promotes pulmonary fibrosis via YY1/FTL axis

doi: 10.1016/j.mtbio.2025.102738

Figure Lengend Snippet: Transcriptional Regulation of FTL by YY1 in PS-NPs-Exposed Lung: (A) Single-cell RNA-seq analysis of FTL expression in alveolar epithelial cells from IPF patients versus healthy controls. (B) FTL mRNA levels in mouse lung tissue after 28 days of PS-NPs exposure (qRT-PCR). (C) Dose-dependent FTL mRNA induction in BEAS-2B cells treated with PS-NPs for 24 h. (D) Actinomycin D chase assay showing FTL mRNA decay kinetics following PS-NPs treatment. (E) Cycloheximide chase assay of FTL protein stability in PS-NPs–treated cells. (F–G) In silico prediction of upstream transcription factors using hTFtarget and PROMO, highlighting YY1 binding motifs in the FTL promoter (JASPAR). (H) Molecular docking simulation of YY1 interacting with the FTL promoter region. (I) ChIP assay validating YY1 binding to the FTL promoter. (J) qRT-PCR analysis of FTL mRNA levels in BEAS-2B cells after siRNA mediated YY1 knockdown. (K) Dual immunofluorescence showing YY1 and FTL co-expression in mouse lung sections (DAPI counterstain; scale bar = 25 μm). (L–M) Western blot and densitometric analysis of FTL protein levels in BEAS-2B cells after siRNA mediated YY1 knockdown. Statistical significance is denoted as *p < 0.05 and **p < 0.01.

Article Snippet: The human bronchial epithelial cell (BEAS-2B) and human embryonic lung fibroblast (MRC-5) were commercially purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: RNA Sequencing, Expressing, Quantitative RT-PCR, In Silico, Binding Assay, Knockdown, Immunofluorescence, Western Blot

Journal: Materials Today Bio

Article Title: Polystyrene nanoplastics-induced lung epithelial cells ferroptosis promotes pulmonary fibrosis via YY1/FTL axis

doi: 10.1016/j.mtbio.2025.102738

Figure Lengend Snippet: PS-NPs Induce YY1 Up-regulation In Vitro and In Vivo: (A–B) Western blot of YY1 in BEAS-2B cells after 24 h treatment with increasing PS-NPs concentrations. (C–D) Time-course analysis of YY1 protein in BEAS-2B cells exposed to 200 μg/ml PS-NPs. (E–F) Immunofluorescence showing nuclear localization and intensity of YY1 in BEAS-2B cells (scale bar = 50 μm). (G) qRT-PCR quantification of YY1 mRNA in BEAS-2B cells across PS-NPs dose gradient. (H) qRT-PCR measurement of YY1 mRNA in lung tissue. (I–J) Western blot and densitometric analysis of YY1 protein in whole-lung lysates. (K–L) IHC detection of YY1 in mouse lung sections following 28 days of PS-NPs exposure. (M − N) Dual-label immunofluorescence for YY1 in SPC + AT2 cells (scale bar = 25 μm). (K) Statistical significance is denoted as *p < 0.05 and **p < 0.01.

Article Snippet: The human bronchial epithelial cell (BEAS-2B) and human embryonic lung fibroblast (MRC-5) were commercially purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: In Vitro, In Vivo, Western Blot, Immunofluorescence, Quantitative RT-PCR

Journal: Materials Today Bio

Article Title: Polystyrene nanoplastics-induced lung epithelial cells ferroptosis promotes pulmonary fibrosis via YY1/FTL axis

doi: 10.1016/j.mtbio.2025.102738

Figure Lengend Snippet: YY1 Silencing Reverses PS-NPs–Induced Ferroptosis and Paracrine Fibrogenesis: (A–C) Western blots showing restoration of GPX4 protein in BEAS-2B cells after YY1 knockdown and 24 h PS-NPs exposure. (D–E) C11-BODIPY581/591 fluorescence demonstrating reduced lipid peroxidation upon YY1 silencing. (F) FerroOrange staining indicating decreased labile Fe 2+ accumulation after YY1 knockdown. (G–H) Double immunofluorescence staining of YY1 with the epithelial marker SPC and the myofibroblast marker α-SMA in lung sections from PS-NPs-treated mice. (I–L) Western blot and qRT-PCR analyses of COL1 and α-SMA in MRC-5 fibroblasts treated for 24 h with conditioned medium from BEAS-2B cells exposed to PS-NPs ± YY1 silencing. Statistical significance is denoted as *p < 0.05 and **p < 0.01.

Article Snippet: The human bronchial epithelial cell (BEAS-2B) and human embryonic lung fibroblast (MRC-5) were commercially purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Knockdown, Fluorescence, Staining, Double Immunofluorescence Staining, Marker, Quantitative RT-PCR